Osteogenic differentiation of amniotic epithelial cells: synergism of pulsed electromagnetic field and biochemical stimuli

Osteogenic differentiation of amniotic epithelial cells: synergism of pulsed electromagnetic field and biochemical stimuli

BACKGROUND Pulsed electromagnetic field (PEMF) is a non-invasive physical therapy used in the treatment of fracture nonunion or delayed healing. PEMF can facilitate the osteogenic differentiation of bone marrow mesenchymal stem cells in vitro. Amniotic epithelial cells (AECs) have been proposed as a potential source of stem cells for cell therapy. However, whether PEMF could modulate the osteog...

Author's response to reviews Title:Osteogenic Differentiation of Amniotic Epithelial Cells: Synergism of Pulsed Electromagnetic Field and Biochemical Stimuli Authors:

Effects of pulsed electromagnetic field frequencies on the osteogenic differentiation of human mesenchymal stem cells.

The purpose of this study was to evaluate the effect of different frequencies of pulsed electromagnetic fields on the osteogenic differentiation of human mesenchymal stem cells. Third-generation human mesenchymal stem cells were irradiated with different frequencies of pulsed electromagnetic fields, including 5, 25, 50, 75, 100, and 150 Hz, with a field intensity of 1.1 mT, for 30 minutes per d...

Effect of pulsed electromagnetic field therapy on the osteogenic and adipogenic differentiation of bone marrow mesenchymal stem cells.

We investigated the effects of pulsed electromagnetic fields (PEMFs) of 20 Hz/2 mT on the osteogenic and adipogenic differentiation of bone marrow stem cells (BMSCs). Sprague Dawley rat BMSCs were isolated and cultured in vitro. The BMSCs of the third passage were obtained and stimulated by PEMFs of 20 Hz/2 mT. The alkaline phosphatase (ALP) activity was measured according to the ALP assay kit ...

Comparison of the effects of extremely low-frequency Electromagnetic field and Betaine on in vitro osteogenic differentiation of human adipose tissue derived-mesenchymal stem cells

Background & Aim: Extremely low-frequency electromagnetic field (ELF-EMF) and betaine are safe factors in bone fracture repair. This study aimed to compare the effects of these two stimuli on osteogenic differentiation of human adipose stem cells (hADSCs). Methods: After obtaining written informed consent, cells were extracted from abdominal adipose tissue and then cultured in vitro until the ...